Aug. 12, 2014
Researchers at the University of Virginia School of Medicine have developed an important new diagnostic approach to determining the cause of diarrhea in the developing world, revealing a problem even more complex than previously thought.
The approach uses a panel of molecular diagnostic assays to pinpoint the cause of the all-too-common illness, diarrhea, the second-leading cause of death in children younger than 5 worldwide.
Often, the researchers have discovered, a child’s symptoms have more than one cause.
“We found diarrhea was caused by multiple infections more often than was appreciated before - often there were two, three or four infections on top of each other,” said Eric Houpt, MD, of the UVa. Division of Infectious Diseases and International Health.
Houpt noted that the new panel of assays offers improved diagnostic ability over the traditional approach. “The old methods missed infections a lot of times,” he said. “There was more Shigella and toxin-producing E. coli than previously seen. It’s as though we are now able to see with glasses.”
“Our methods were able to not just detect infections but quantify how much was there, and that proved to be critical,” Houpt said. “In these impoverished parts of the world, there’s enormous exposure to different bacteria, viruses and parasites, and you can find low amounts with these sophisticated methods. We were able to figure out the right amount above which it becomes clinically relevant – that is, that it’s the likely cause of diarrhea.”
The researchers have already put their new approach to the test, deploying it across three platforms to five institutions around the world – two in Africa and three in Asia. The researchers say their approach has proved more sensitive and less expensive than traditional testing, while providing more holistic information about the causes of childhood diarrhea.
The new approach could be useful for other illnesses as well. “It’s not just for diarrhea. We are applying it to other infectious diseases as well,” said Jie Liu, PhD, the lead author of a new paper outlining the approach and the results of its testing. “We look forward to expanding this into a bigger study.”
The new approach has important applications in the U.S. as well, offering an easy way to detect pathogens without the need to run multiple tests for specific types.